ab18192 rabbit polyclonal anti phosphorylated atr ser428 cell signaling technology  (Cell Signaling Technology Inc)

Journal: Frontiers in Physiology

Article Title: Inositol-requiring enzyme 1 alpha is essential for dentinogenesis

doi: 10.3389/fphys.2025.1722417

Figure Lengend Snippet: Immunohistochemistry staining of phosphorylated IRE1α (pIRE1α) protein in the mandibular first molars. (A) Shown are the representative images of immunohistochemistry staining of pIRE1α protein (signal in brown) in the mandibular first molars of 3-week-old Dspp +/+ , Dspp P19L/+ , and Dspp P19L/P19L mice. Each image in (A) is from the middle region of the crown of a sagittally-sectioned mandibular first molar. (A1-A3) are the higher magnification views of the roof-forming odontoblasts (box1), dental pulp cells (box 2) and floor-forming odontoblasts (box 3) in the corresponding images in (A) , respectively. rd, roof dentin; fd, floor dentin; rod, roof-forming odontoblasts; fod, floor-forming odontoblasts; Scale bars: 50 μm in A; 20 μm in (A1-A3). Three independent experiments for IHC staining of pIRE1α show similar results.

Article Snippet: The primary antibodies used in this study included: 1) rabbit anti-phosphorylated IRE1α polyclonal antibody (1:400, Novus Biologicals, NB100-2323); 2) rabbit anti-XBP1 polyclonal antibody that recognizes both unspliced XBP1 (XBP1U) and spliced XBP1 (XBP1S) (1:200, Abcam, ab37152); 3) rabbit anti-XBP1S monoclonal antibody (E9V3E) that specifically recognizes XBP1S ( ; ) (1:50, Cell Signaling Technology, Danvers, MA); 4) rabbit anti-DSPP polyclonal antibody that recognizes both DSP and full-length DSPP (1:2000) ( ); and 5) rabbit anti-DMP1 polyclonal antibody (1:800, #857) ( ).

Techniques: Immunohistochemistry, Staining

Journal: The American Journal of Pathology

Article Title: Hyperactivation of Transforming Growth Factor-β–Activated Kinase 1 Causes Skeletal Muscle Pathology Reminiscent of Inflammatory Myopathies

doi: 10.1016/j.ajpath.2025.07.005

Figure Lengend Snippet: High TAK1 activity stimulates catabolic signaling in adult skeletal muscle. Immunoblots ( A ) and densitometry analysis ( B ) for protein levels of phosphorylated IκBα (p-IκBα), IκBα, phosphorylated p65 (p-p65), p65, p100-p52, phosphorylated c-Jun N-terminal kinase (p-JNK), total JNK, phosphorylated p38 mitogen-activated protein kinase (p-p38 MAPK), and total p38 MAPK in gastrocnemius (GA) muscle expressing high levels of green fluorescent protein (GFP) or TAK1/TAB1 protein. Immunoblots ( C ) and densitometry analysis ( D ) of phosphorylated AMP-activated protein kinase α (p-AMPKα), AMPKα, phosphorylated STAT3 (p-STAT3), and STAT3 protein in GA muscle expressing high levels of GFP or TAK1/TAB1 protein. Immunoblots ( E ) and densitometry analysis ( F ) of phosphorylated mammalian target of rapamycin (p-mTOR), mTOR, phosphorylated p70S6K1 (p-p70S6K1), p70S6K1, phosphorylated rpS6 (p-rpS6), rpS6, phosphorylated 4EBP1 (p-4EBP1), 4EBP1, and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) protein in GA muscle expressing high levels of GFP or TAK1/TAB1 protein. All data are presented as means ± SEM ( B , D , and F ). n = 6 mice in each group ( A – F ). ∗ P ≤ 0.05, values significantly different from contralateral muscle expressing GFP analyzed by unpaired t- test.

Article Snippet: Polyclonal rabbit–anti-phosphorylated mTOR , Cell Signaling Technology/2971 , 1:500.

Techniques: Activity Assay, Western Blot, Expressing